Biostratigraphy is frequently used to generate age models and is significant to understanding the rate and timing of Cenozoic climate change. Records from the Southern Ocean (SO) are particularly valuable in understanding the past behavior of the Antarctic Ice Sheet, whereby clues to this behavior can be gained from the presence and composition of preserved microfossils. Diatoms, a nearly ubiquitous group of microalgae that make cell walls out of opal, preserve well in Southern Ocean sediments and have been used extensively in Southern Ocean biostratigraphy. Here, we present an updated diatom biostratigraphy of the Southern Ocean extending 3.3 Myr from sediments recovered during International Ocean Discovery Program (IODP) Expedition 382 “Iceberg Alley” Site U1537. Furthermore, we compare a tuned age model to a paleomagnetic-based age model to provide two independent estimates of ages of these datums with quantified uncertainty. The high sedimentation rate found at Site U1537 allows detailed age assessment, allowing the generation of more finely tuned age models in Southern Ocean sediments.