The Bohemian Massif hosts significant hydrothermal U-deposits associated with shear zones in the high-grade metamorphic basement. But there is a lack of evidence of a genetic link between mineralization and U-fertile igneous rocks. This contribution provides constraints on the major U source of the vein-type U-deposits, the timing of ore formation and the metallogenetic model. The anomalous trace element signatures of the low-temperature hydrothermal deposits (high Zr, Y, Nb, Ti, Sigma REE) and their close spatial relation with ultrapotassic rocks of the durbachite series point to a HFSE and REE enriched source rock. The durbachites have high U content (13.4-21.5 ppm) mainly stored in magmatic uraninite and other refractory minerals (e.g., thorite, zircon, allanite) that became metamict over a time interval sufficient to release U from their crystal structure, as suggested by the time gap between emplacement of the durbachites (EMP uraninite U-Pb age similar to 338 Ma) and hydrothermal activity (SIMS uranium ore U-Pb age similar to 270 Ma). Airborne radiometric data show highly variable Th/U ratios (1.5-6.0), likely reflecting a combination between (1) crystallization of magmatic uraninite, (2) hydrothermal alteration, and (3) leaching and mobilization of U along NW-SE-trending fault zones, manifested by elevated Th/U values in the radiometric map. The presence of rare magmatic uraninite in durbachites suggests almost complete uraninite dissolution; EMP imaging coupled with LA-ICP-MS analyses of refractory accessory phases revealed extensive mobilization of U together with HFSE and REE, providing direct evidence for metal leaching via fluid-driven alteration of radiation-damaged U-rich minerals. The large-scale HFSE and REE mobilization, demonstrated by the unusual trace element signatures of the U-deposits, was likely caused by low-temperature (270-300 degrees C), highly alkaline aqueous solutions containing F-, P-, and K-dominated complexing ligands. The first SIMS U-Pb age of 270.8 +/-; 7.5 Ma obtained so far for U-mineralization from the Bohemian Massif revealed a main Permian U mineralizing event, related to crustal extension, exhumation of the crystalline basement, and basin formation, as recorded by U-Pb apatite dates (280-290 Ma) and AFT thermal history models of the durbachites. The Permo-Carboniferous sedimentary cover probably represented a source of oxidized basinal brines infiltrating the basement-hosted durbachite plutons and triggering massive metal leaching. The interaction between basin-derived brines and durbachites resulted in significant modification of the chemical composition of the hydrothermal system (K and F release during biotite chloritization, P liberation through monazite alteration), leading to the formation of ore-bearing fluids responsible for the metallogenesis of the basement-hosted unconformity-related U-deposits in shear zones in the Bohemian Massif.