The Changjiang uranium (U) orefield in northern Guangdong (South China) contains several important graniterelated uranium deposits, including the Mianhuakeng deposit. The hydrothermal evolution and mineralization mechanism of the deposit remain unclear, and hence in this study we analyzed the mineral compositional variations of pitchblende from different elevation (-300 to -50 m) in the orebody at Mianhuakeng deposit. The results indicate that with decreasing depth, the hydrothermal mineral assemblage changes from a reducing one (pitchblende, pyrite, and chlorite) to a moderately oxidizing one (pitchblende, coffinite, hematite, and goethite). The contents of U, Sr, & sum;REE, U/Th, and LREE/HREE ratios in the pitchblende decrease (whereas the Cu-Pb-ZnNi-Co-Sc-Rb contents increase) progressively with decreasing depth. Also, Ce anomaly (Ce/Ce*) changes from positive to negative, and Eu anomaly (Eu/Eu*) becomes more negative with decreasing depth. We interpreted such vertical variations to be caused by the ascent of deep, mid-to-high temperature, highly-oxidizing oreforming fluid, which was reduced by early-stage reducing minerals in the wallrock, a process that also formed the mineralization at Changjiang. In addition, the pitchblende REE distribution patterns and Eu/Eu* at depth (-300 m) resemble those of the Zhuguang pluton, indicating that the ore-forming materials were originated from the Ubearing granite wallrocks. Therefore, the Ce/Ce* and Eu/Eu* variations of pitchblende can be used to guide deep uranium ore exploration.