There are a lot of exploration examples of far-source reservoirs, but the description of the connotation and accumulation mechanism is very rare. The Dongsha Uplift is one of the major oil-producing areas in the eastern Pearl River Mouth Basin in the northern South China Sea. The uplift lacks generally hydrocarbon source rock and is far from the generative kitchen, making it a typical far-source oil and gas reservoir. The accumulation mechanism of crude oil in the reservoirs are still unknown. By systematically comparing biomarkers, nitrogenous compounds and isotopic characteristics of the crude oil and source rocks with those in the neighboring depressions, and combining with fracture, sand body, unconformity migration conduits and barrier conditions, this paper simulates oil and gas migration paths and formation time with Pathway and IES software, and analyzes oil and gas accumulation process from a dynamic perspective. We found that the deep lacustrine hydrocarbon source rocks of the Wenchang Formation in the H26 Sag of the Huizhou Depression reached peak oil production at the end of the Hanjiang Formation deposition. Influenced by the strong tectonic activities at the final deposition of the Hanjiang and Yuehai formations, two episodes of hydrocarbon charging occurred at the top of the Dongsha Uplift. The accumulation of hydrocarbons far from the source rocks in the Dongsha uplift is mainly controlled by the efficient carrier system at hydrocarbon generation period. The oil is mainly accumulated in L4-1 and L11-1 oil fields through the long-distance stepped migration mode. The spatial and temporal relationship between hydrocarbon generation of source rock and episode of fault activity are mainly responsible for accumulation in the far-source reservoirs of the Dongsha Uplift. • Under the influence of strong tectonic activities, two episodes of oil and gas charging took place in the far-source reservoirs. • The hydrocarbons migrated horizontally to the far-source reservoirs through the stepped compound carrier system composed of sandstone and faults. • The spatial and temporal relationship between hydrocarbon generation of source rock and episode of faulting are mainly responsible for accumulation in the far-source reservoirs.