Paleolatitude evolution could provide a general paleo-location framework for explaining the paleoclimate change and tectonic deformation in geological time. Strengthening the paleolatitude study of the Tuotuohe Basin is important for understanding the history and mechanism of the tectonic uplift process in the north-central Tibetan Plateau. In this study, we introduced the magnetostratigraphy for the Tuotuohe-D (TTH-D) section in the Tuotuohe Basin, central-northern Tibetan Plateau, in order to constrain the chronology and to reconstruct the paleolatitude of the basin during the deposition of the Tuotuohe Formation. The results indicated that the Tuotuohe Formation in the TTH-D section was deposited between 38.5 and ~36.7 Ma. Combining this age with the results from the Tuotuohe section indicates that the age of the Tuotuohe Formation spans the interval from >38.5 Ma to ~33 Ma. Additionally, other paleomagnetic data of the Tuotuohe Formation from the Tuotuohe section, combined with the data from this study, indicate that the paleolatitude of the Tuotuohe Basin during the late Eocene was 25.9 ± 4.2°. That means that the Tuotuohe Basin was located in a subtropical anticyclonic zone and that the paleoenvironment during the late Eocene might be controlled by subtropical high pressure. Additionally, paleomagnetic results from the Qiangtang terrane and the bordering regions are combined with the results of our study, which suggest that the paleolatitude of the Tuotuohe Basin at ~26 Ma coincides well with the Eurasian apparent polar wander path for that interval, and that the N-S India–Asia convergence was reduced or ceased at ~26 Ma in the Tuotuohe Basin.